Pathophysiology characterization of Alzheimer’s disease in South China’s aging population: for the Greater-Bay-Area Healthy Aging Brain Study (GHABS)

Introduction The Guangdong-Hong Kong-Macao Greater-Bay-Area of South China has an 86 million population and faces a significant challenge of Alzheimer’s disease (AD). However, the characteristics and prevalence of AD in this area are still unclear due to the rarely available community-based neuroimaging AD cohort. Methods Following the standard protocols of the Alzheimer’s Disease Neuroimaging Initiative, the Greater-Bay-Area Healthy Aging Brain Study (GHABS) was initiated in 2021. GHABS participants completed clinical assessments, plasma biomarkers, genotyping, magnetic resonance imaging (MRI), β-amyloid (Aβ) positron emission tomography (PET) imaging, and tau PET imaging. The GHABS cohort focuses on pathophysiology characterization and early AD detection in the Guangdong-Hong Kong-Macao Greater Bay Area. In this study, we analyzed plasma Aβ42/Aβ40 (A), p-Tau181 (T), neurofilament light, and GFAP by Simoa in 470 Chinese older adults, and 301, 195, and 70 had MRI, Aβ PET, and tau PET, respectively. Plasma biomarkers, Aβ PET, tau PET, hippocampal volume, and temporal-metaROI cortical thickness were compared between normal control (NC), subjective cognitive decline (SCD), mild cognitive impairment (MCI), and dementia groups, controlling for age, sex, and APOE-ε4. The prevalence of plasma A/T profiles and Aβ PET positivity were also determined in different diagnostic groups. Results The aims, study design, data collection, and potential applications of GHABS are summarized. SCD individuals had significantly higher plasma p-Tau181 and plasma GFAP than the NC individuals. MCI and dementia patients showed more abnormal changes in all the plasma and neuroimaging biomarkers than NC and SCD individuals. The frequencies of plasma A+/T+ (NC; 5.9%, SCD: 8.2%, MCI: 25.3%, dementia: 64.9%) and Aβ PET positivity (NC: 25.6%, SCD: 22.5%, MCI: 47.7%, dementia: 89.3%) were reported. Discussion The GHABS cohort may provide helpful guidance toward designing standard AD community cohorts in South China. This study, for the first time, reported the pathophysiology characterization of plasma biomarkers, Aβ PET, tau PET, hippocampal atrophy, and AD-signature cortical thinning, as well as the prevalence of Aβ PET positivity in the Guangdong-Hong Kong-Macao Greater Bay Area of China. These findings provide novel insights into understanding the characteristics of abnormal AD pathological changes in South China’s older population. Supplementary Information The online version contains supplementary material available at 10.1186/s13195-024-01458-z.

The prevalence of dementia and mild cognitive impairment (MCI) among older adults aged 60 and above in China is 6.0% and 15.5%, respectively.Among those cases, the majority (65%) is dementia or MCI due to AD, followed by vascular dementia (26.7%) and other dementias (8.3%) [24].Additionally, the prevalence of preclinical AD defined by Aβ PET imaging among adults aged 60 or older was around 18% [21].Approximately 260 million individuals are 60 years or older in China [25].Among them, around 15 million, 39 million, and 47 million individuals are at the stage of dementia, MCI, and preclinical AD, respectively.China's total expenditure on dementia treatment and nursing services will be $1.89 trillion around 2050 [26].AD has become the fifth leading cause of death disease in China in 2019 [27].The agestandardized prevalence and the age-standardized death rate of AD and related dementias were 788.3/100 000 and 23.3/100 000, separately, which were slightly higher than that of the global levels (682.5/100000 and 22.9/100 000, separately) [27].The high prevalence and vast population of AD severely challenge China.
Recently, three anti-Aβ drugs, including Aducanumab [28], Lecanemab [29], and Donanemab [30] showed positive results in phase 3 clinical trials.Consequently, early AD diagnosis and intervention are critical for preventing AD progression.However, the standardization of biomarker measurements, magnetic resonance imaging (MRI), and PET image scanning and processing of AD are not fully established yet in China.Therefore, the neuroscientists, neurologists, pathologists, radiopharmacists, biomedical engineers, and biochemists in Guangdong-Hong Kong-Macao Greater-Bay-Area are working together to initiate the Greater-Bay-Area Healthy Aging Brain Study (GHABS), aiming to investigate the pathological features and progression patterns of AD, especially in asymptomatic stage of AD.GHABS participants will undergo clinical neuropsychological assessments, biospecimen sample collection, MRI imaging, and PET imaging.The GHABS project aims to: (1) explore the risk factors of Aβ and tau aggregation in the early stage of AD among China's aging population; (2) determine the effect of Aβ and tau pathologies upon downstream neurodegeneration and cognitive decline in both Aβ negative (Aβ-) and Aβ positive (Aβ+) elderly adults; (3) identify novel approaches and techniques for early detection of AD and provides significant reference for the target brain region and appropriate time window for anti-AD treatments.
In this study, we first summarized the aims, study design, data collection, and potential applications of GHABS.Second, we compared the plasma Aβ 42 /Aβ 40 , plasma p-Tau 181 , plasma GFAP, plasma NfL, Aβ PET, tau PET, hippocampal volume, and temporal-MetaROI cortical thickness between normal control (NC), subjective cognitive decline (SCD), MCI, and dementia groups.Third, we also reported the prevalence of plasma A/T staging and Aβ PET positivity of different diagnostic groups in the Guangdong-Hong Kong-Macao Greater-Bay-Area of South China.

Study design
Shenzhen Bay Laboratory launched the communitybased longitudinal cohort study GHABS (ClinicalTrials.govID: NCT06183658) in May 2021.The GHABS project was approved by the Shenzhen Bay Laboratory's and the collaborated hospitals' Ethical Committees.The flow chart of participant engagement in the GHABS is delineated in Supplementary Fig. 1.The GHABS participants were recruited via posters and lectures in the community and nursing homes.Each participant signed the written informed consent of the GHABS project before enrollment.The participants who met the inclusion and exclusion requirements were informed about the baseline and follow-up examinations.From 2021 to 2026, the GHABS cohort will recruit 1400 individuals aged 55 and older, including 1100 CU older adults, 200 MCI patients, and 100 dementia patients.The scheme for recruiting GHABS participants is illustrated in Supplemental Fig. 1.
All the GHABS participants will undergo cognitive assessments, genetic screening, and blood sample collection.Some will have CSF collection, stool sample collection, MRI scanning, Aβ PET scanning, and tau PET scanning.All baseline examinations will be completed within three months.At follow-up, clinical assessments and blood sample collection will be conducted annually.CSF sample collection, MRI scan, Aβ PET scan, and tau PET scan are evaluated every two years.Six hundred twenty-seven participants have completed cognitive assessments and blood sample collection in the GHABS cohort by Sep 08, 2023 (Fig. 1).Among them, 26%, 44%, 19%, and 11% of the cohort were NC, SCD, MCI, and dementia, respectively.Additionally, 369 and 93 participants completed stool and CSF sample collection.So far, 469, 370, and 105 participants had MRI, Aβ PET, and tau PET imaging scans, respectively.This study identified 470 GHABS participants who simultaneously completed cognitive assessments, plasma Aβ 42 /Aβ 40 , p-Tau 181 , NfL, and GFAP data measured by the Simoa platform.Among them, 119, 207, 87, and 57 were NC, SCD, MCI, and dementia.Moreover, 301, 195, and 70 individuals had concurrent MRI, 18 F-D3-FSP Aβ PET, and FTP tau PET scans.

Inclusion and exclusion criteria
Briefly, the inclusion criteria of GHABS are as follows: (1) adults between the ages of 55 and 90 and speak Mandarin fluently [31]).Notably, individuals below 60 years old are required to have a family dementia history and meet the criteria of subjective cognitive decline (SCD), while people with family history of autosomal dominant or other familial AD are not limited by age; (2) the score on the Geriatric Depression Scale (GDS) is less than 6 points; (3) visual and auditory acuity is sufficient for neuropsychological testing (Including normal corrected vision and hearing); (4) female participants are not pregnant, lactating, or of childbearing potential (that is, women must be two years postmenopausal or surgically sterile); (5) a modified version of the Hachinski Ischemic scores less than or equal to 4; (6) have completed primary school (6 years of education) or have good work experience (sufficient to rule out mental retardation).Individuals with an infection, infarction, or other focal lesions or multiple lacunes or lacunes in critical memory structures and who do not meet the MRI scanning requirements are excluded from the GHABS study.More details of the inclusion and exclusion criteria can be found in the supplemental materials.

Cognitive assessments
The The participants were classified as NC, MCI, or dementia due to AD following the standard protocol of ADNI cohort [35].We further define some CU individuals as SCD following the research criteria proposed by Jessen and colleagues in 2014 [36].Several cognitive assessments were used for clinical diagnosis.The normal performance of these assessments was defined based on education or age.The MMSE used cutoff scores: >17 for participants without education, > 20 for 1-6 years of education, and > 24 for more than six years of education.The delayed recall of logical Memory test used cutoff scores as follows: ≥3 for 0-7 years, ≥5 for 8-15 years, and ≥9 for 16 years of education [37].As for the ADL, < 23 is normal for participants under 75 years old, while < 25 is for 75 years and older.
CU participants were normal in MMSE, logical Memory recall, and ADL, and their CDR score was 0. Among CU individuals, the presence of the following symptoms as having SCD: (1) Self-experienced persistent decline in cognitive capacity in comparison with a previously normal status and unrelated to an acute event; (2) Normal age-adjusted, gender-adjusted and education-adjusted performance on standardized cognitive tests.CU individuals without SCD were defined as the NC group.
Participants with MCI had unimpaired MMSE, while they showed impairment in the logical Memory test [38].Their CDR score was 0.5, with a mandatory requirement of the memory box score being 0.5 or greater, but ADL was normal.Dementia due to AD was abnormal in MMSE, logical memory, and ADL.The CDR score was 0.5 or greater.

Biospecimen collection
Volunteers fasted for one night the day before (not less than 6 h), and blood was drawn in the morning of the next day.The venous blood of the volunteers is drawn into two 10 ml EDTA blood collection tubes and gently inverted and mixed 10-12 times to ensure that the blood and anticoagulant are thoroughly mixed.The mixed blood was placed in an incubator at 4 °C and shipped back to the laboratory within 4 h for subsequent analysis.The blood is centrifuged at 1600 g for 10 min in a refrigerated centrifuge at 4 °C.The upper plasma layer is transferred to several 2 ml centrifuge tubes using a sterile RNase-free pipette tip.To obtain more pure plasma, the separated plasma is centrifuged again at 16,000 g for 10 min at 4 °C, and then the supernatant is aliquoted into several 0.5 ml centrifuge tubes with labels, each with either 100-200 µl blood plasma.The aliquots are stored in a -80 °C refrigerator for subsequent analysis.After the whole blood is centrifuged in the first step, the buffy coat in the middle layer is gently transferred to the 2640 medium.Then, after density gradient centrifugation, erythrocyte lysis, and centrifugation steps, the isolated peripheral blood mononuclear cell sample is transferred to a 2 ml RNase-free centrifuge tubes and stored in a gradient-cooled freezer box at -80 °C for subsequent analysis.Samples will be used for genomic analysis (including whole genome sequencing and other analyses).
Before collecting the CSF sample, the volunteer must fast for one night (at least 6 h), and the lumbar spinal fluid is performed on an empty stomach the following day.Lumbar puncture is performed strictly with clinical standards, and about 5 ml of CSF is collected.The collected CSF is sent to Shenzhen Bay Laboratory within 2 h for biomarker analysis.CSF sample is quickly divided into 1.5 ml low protein adsorption centrifuge tubes.Afterward, they will be stored in a -80 °C refrigerator for subsequent analysis.
Fecal samples are collected on-site or at home after the volunteers' consent is obtained.The volunteer stool samples are labeled, sub-packaged, and frozen in a -80 °C refrigerator.Fecal samples are used for 16 S rDNA, metagenomic, metagenome, and metabolome detection of intestinal microorganisms.
The concentrations of PDGFR-β and GAP43 in CSF and plasma are measured by commercial Human PDGFR-β (R&D, Catalog Number: DYC385) and GAP43 (Abbexa) ELISA kits.For neurogranin, an in-house sandwich ELISA is developed combining the mouse monoclonal antibodies Ng22 and Ng2 (Abcam).Nunc maxisorp 96-well microliter plates (Thermo Fisher Scientific) are coated with the mouse anti-human neurogranin (Ng22, 3 µg/mL) in bicarbonate buffer pH 9.6 overnight.After washing with PBS containing 0.05% Tween-20, the plates are blocked in PBS containing 1% BSA and 0.05% Tween-20 for 1 h, followed by incubation with CSF and plasma samples overnight.Recombinant full-length human neurogranin protein is used as a standard (25 to 3200 pg/ ml for CSF, 55 to 40,000 pg/mL for plasma).After additional washes, the plates are incubated with the biotinylated mouse anti-human neurogranin (Ng2, 2.7 µg/mL) for 1 h, followed by incubation with a Streptavidin-HRP (R&D Systems) for 30 min.After additional washes, the plates are incubated with Substrate Reagent (TMB, R&D Systems) for 10 min in the dark.The color reaction is stopped by adding 0.2 M H 2 SO 4, and the absorbance is read at 450 nm (650 nm as a reference value).
All samples were analyzed randomly and doubleblindly to avoid bias because of the effect of inter-assay variability on specific patient groups.For all biomarker measurements, the samples in the first plate were tested in duplicate, and subsequent samples were tested in single.A pooled sample was generated as a reference, aliquoted and stored at -80 °C.In each measurement, the reference sample was tested in duplicate to compare the detection variability.The mean intra-assay and interassay coefficient of variation (CV) was controlled within 20%.

MRI image acquisition
All the MRI scanning sequences will be conducted following the standard ADNI protocol here.The MRI image data is collected on 3.0T scanners, and the scanning parameters vary slightly depending on the specifics of scanners from various clinical centers.A series of sequences are applied for head imaging of each volunteer, including3D T1 MPRAGE, 3D T2 FLAIR (Fluid-Attenuated Inversion Recovery), High Res Hippo (High Resolution Hippocampus), SWI (Susceptibility Weighted Imaging), ASL (Arterial spin labeling), Diffusion (Axial DTI), Field Mapping, EPI-BOLD.Each sequence and parameters of MRI scanning are described as follows: 3D

PET image acquisition
The Aβ PET radiotracer [ 18 F]-florbetapir (FBP) [40] or [ 18 F]D3FSP (FSP) [41] and tau PET radiotracer [ 18 F]-flortaucipir (FTP) [42] are used for PET imaging.The data acquisition is performed on either a GE Dis-covery™ MI Gen 2 PET/CT scanner or a Siemens Bio-graph™ TruePoint™ TrueV PET/CT scanner.The spatial resolution of each PET scanner is quantified with PET imaging of a Hoffman phantom.For the Aβ PET imaging, the subjects are injected with either [ 18 F]-florbetapir or [ 18 F]-D3FSP intravenously at 370 MBq (10 mCi ± 10%), rested for 45 min and prepared for the scanning.PET/ CT imaging is performed 50 min after injection, and the PET acquisition time is 20 min.For the tau PET imaging, the participants are injected with [ 18 F]-flortaucipir intravenously at 370 MBq (10 mCi ± 10%), rested for 75 min, and prepared for imaging.The dynamic acquisition of [ 18 F]-flortaucipir tau PET data is completed 80-100 min after the radiotracer administration.
A dedicated head scanning procedure covering the whole brain from vertex to cerebellum is used for imaging.A diagnostic dose CT scan of the brain is acquired beforehand for attenuation correction and fusion localization of PET images.The PET scans are acquired using 3D list mode on the GE Discovery MI and Siemens BioGraph TruePoint scanners in two sites.For the GE scanner, the FOV is 256 mm×256 mm×220 mm, the scanning matrix is 336 × 336 × 109, and the voxel size is 1.02 mm×1.02mm×2.03mm.For the Siemens scanner, FOV is 256 mm×256 mm×198 mm, the scanning matrix is 192 × 192 × 71, and the voxel size is 1.33 mm×1.33 mm×2.79 mm.All the correction options were selected for both scanners, and no filter or smooth was used during the reconstruction.A reconstruction offset is applied to ensure that the head is entirely in the field of view within the plane.Finally, 4 frames of dynamic images are generated according to 5 min/frame segmentation, with each PET scan corresponding to a 20-minute PET image.

MRI and PET imaging analysis
The structural MRI images are segmented into different cortical and subcortical regions of interest (ROI) in Freesurfer (V7.2.0).The residual hippocampal volume (rHCV) is calculated using the hippocampal volume of both hemispheres and adjusted using the estimated total intracranial volume as we described previously [14].In addition, the cortical thickness of AD-signature atrophy brain regions is obtained by calculating the surface areaweighted average thickness of the bilateral entorhinal, fusiform, inferior temporal, and middle temporal cortices [43].
As shown in Fig. 2, the PET images are preprocessed with the following steps before further analysis: (1) coregistering the 2nd, 3rd, and 4th frames to the 1st frame, respectively; (2) averaging the four frames into one averaged frame; (3) the averaged frame resliced into a standard AC-PC space (anterior commissure-posterior commissure) with image size = 160 × 160 × 128, voxel dimension = 1.5 mm×1.5 mm×1.5 mm; (4) this standardized image then served as a reference imaging, and each Fig. 2 The image processing pipelines of PET, structural MRI, functional MRI (fMRI), and diffusion MRI data of the Greater-Bay-Area Healthy Aging Brain Study original individual frame was resliced to this reference imaging; (5) smoothing to a uniform 6 mm resolution using the Gaussian kernel function, and the full-width at half maximum (FWHM) of each site was determined from the Hoffman phantom [ 18 F]FDG-PET imaging (As a reference for mitigating between-scanner differences in multi-center PET scans).The PET and MRI images are processed using in-house Matlab algorithms, as shown in Fig. 2. The PET images are co-registered with their corresponding structural MRI images in SPM12 (Statistical Parametric Mapping).Sixty-eight Freesurfer-defined cortical ROIs obtained from MRI segmentation extract regional FSP, FBP, and FTP measurements from the coregistered PET images.
The FSP and FBP standardized uptake value ratio (SUVR) of AD summary cortical regions (posterior cingulate cortex, precuneus, frontal lobe, parietal lobe, and lateral temporal) are obtained by dividing the radiotracer uptake value of AD typical brain regions by that in the whole cerebellum [44].In the present study, we only presented FSP Aβ PET data.For the [ 18 F]-flortaucipir images, FTP SUVR of 68 FreeSurfer-defined ROIs are calculated by normalizing the [ 18 F]-flortaucipir value to the value of the inferior cerebellar cortex [45].The FTP SUVR of the AD Temporal-MetaROI [43] (entorhinal cortex, parahippocampal gyrus, fusiform, amygdala, inferior temporal and middle temporal brain regions) is used to evaluate cortical tau deposition.
The general process of resting-state fMRI data preprocessing and brain functional connectivity construction are outlined in the third column of Fig. 2 and described in detail in the Supplemental Materials.The general process of diffusion MRI processing are outlined in the fourth column of Fig. 2 and described in detail in the Supplemental Materials.Diffusion-weighted data are denoised and corrected for Gibbs ring using Mrtrix3 (V3.0.3) [46], and then corrections were applied for head motion, eddy current, and EPI susceptibility distortion using FSL (V6.0.3) [47].3D pCASL imaging was employed to calculate the CBF map (Supplemental Fig. 3).The WMH segmentation was processed using a custom pipeline developed by our lab based on the T2 FLAIR images (Supplemental Fig. 4).More details of fMRI, dMRI, WMH, and pCASL can be found in Supplementary Material.

Statistical analysis
All the statistical analyses were conducted using R (v4.3.0,The R Foundation for Statistical Computing).The normal distribution of the data in this study was determined using the Shapiro-Wilk test.Plasma p-Tau 181 , plasma GFAP, and plasma NfL were log 10 transferred before the following analysis to meet the normal distribution.We used a two-tailed Mann-Whitney U test and Fisher's exact test to compare the continuous and categorical characteristics at baseline between different diagnosis groups, respectively.Data were presented as median (interquartile range, IQR) or No. (%) unless otherwise noted.Generalized linear models were used to compare plasma biomarkers, COMPOSITE FSP Aβ SUVR, temporal-metaROI FTP SUVR, rHCV, and temporal-metaROI cortical thickness between different clinical groups, controlling for age, sex, and APOE-ε4.Subsequently, we investigated the frequency of different A/T profiles defined by plasma biomarkers (A: plasma Aβ 42 /Aβ 40 , T: plasma p-Tau 181 ) and Aβ PET positivity among NC, SCD, MCI, and dementia groups.

Demographics of participants
The demographic characteristics of participants included in this study are summarized in Table 1.At baseline, MCI and dementia individuals had older ages, higher percentages of APOE-ε4 carriers, shorter duration of education, and lower MoCA and MMSE scores than NC and SCD individuals.The dementia group also had lower MoCA and MMSE scores than the MCI group.The SCD group had more females than the MCI and dementia groups.Additionally, 443 participants had risk factors records, including the medical history of hypertension, hyperlipidemia, and diabetes, as well as the assessments of PSQI and GDS.SCD individuals had higher percentage of hyperlipidemia compared to the NC and MCI groups.SCD and MCI individuals had worse sleep state than NC and dementia individuals, while dementia individuals showed better sleep state than NC individuals.MCI and dementia individuals had higher depression scores than SCD and NC individuals, whereas SCD individuals showed higher depression scores than NC individuals.The demographic data of the GHABS cohort by cities are summarized in Supplemental Tables 2 and have been compared with the corresponding demographcis from the literature, as presented in Supplemental Table 3 [48,49].

Discussion
There are approximately 47 million preclinical AD individuals, 39 million MCI patients, and 15 million dementia patients in China.In 2035, the older population aged 60 and above is expected to exceed 400 million, accounting for more than 30% of the total population.As long as there are no practical methods for the early detection and treatment of AD, the number of AD patients in China will continue to rise.To end this, the GHABS study was initiated in 2021.The GHABS study aims to investigate the prevalence and progression of AD in the Guangdong-Hong Kong-Macao Greater-Bay-Area of China.The ultimate goal is to develop novel biomarkers and neuroimaging approaches for early diagnosis of AD and support the early intervention of clinical trials in the Guangdong-Hong Kong-Macao Greater-Bay-Area (Fig. 5).
Following the standard protocol of ADNI [50], the GHABS is supposed to be a high-standard AD community cohort in the Guangdong-Hong Kong-Macao Greater-Bay-Area of China.GHABS referred to several well-established large-scale AD cohorts from Europe and the United States, such as the Mayo Clinical Study of Aging [51], the Harvard Aging Brain Study [52], the Swedish Biomarkers For Identifying Neurodegenerative Disorders Early and Reliably, the Amyloid Imaging to Prevent Alzheimer's Disease [53], and the Australian Imaging, Biomarkers and Lifestyle Study [54].Based on these databases, many breakthroughs have been made in AD-related gene loci, biomarker detection, PET molecular imaging, etc., which provide important support for screening target participants and therapeutic targets in AD clinical trials.Besides, GHABS also referred to several well-organized cohorts in China, such as the Beijing Aging Brain Rejuvenation Initiative [55], China Aging and Neurodegenerative Initiative [56], Chinese Alzheimer's Biomarker and LifestylE [57], the Chongqing Ageing & Dementia Study (CADS) [58], Chinese Preclinical Alzheimer's Disease Study (C-PAS) [59], the China Cognition and Ageing Study [60], the Taizhou Imaging Study [61], and Sino Longitudinal Study on Cognitive Decline [62].Dr. Ying Han, the president of the "Pre-Alzheimer's Disease Alliance of China, " is one of the principal investigators in GHABS.Dr. Han initiated the Pre-AD Alliance of China in 2017 [63] and led two AD cohorts focused on the population of SCD for early AD diagnosis and investigation, the SILCODE and Cross-Cultural Longitudinal Study on Cognitive Decline [64].The design of GHABS will be updated by the researchers if necessary.GHABS closely follows the latest academic and industry developments in the field and tries to adapt and update protocols.
The primary goal of GHABS is to investigate the pathological characteristics, risk factors, protective indicators, and evolution of Aβ and tau pathologies in Guangdong-Hong Kong-Macao Greater-Bay-Area older adults.The specific studies are as follows: (1) summarize the incidence of preclinical AD (Aβ + CU) in the Guangdong-Hong Kong-Macao Greater-Bay-Area, and reveal the Compared to CSF biomarkers and PET imaging, plasma biomarkers are the most promising early screening technology for AD by considering the advantages of simple sampling, low traumatic, and cost [65].Recently, plasma Aβ 42 /Aβ 40 [66,67], p-Tau 181 [68], p-Tau 217 [69], p-Tau 231 [70], and GFAP [71] showed great potential in early diagnosis of AD.One of the primary goals of GHABS is to evaluate the performance of previously reported plasma biomarkers and further explore novel plasma biomarkers in China's aging population.For example, the GHABS research group has investigated the characteristics of CSF GAP43 in different clinical and pathological stages of AD [11] based on the ADNI cohort and demonstrated that presynaptic dysfunction measured by CSF GAP43 occurs prior to AD typical neurodegeneration and predicts faster cognitive decline [12].We are currently measuring GAP43 concentrations in plasma in the GHABS cohort and evaluating its suitability as a plasma synaptic biomarker of AD in the Chinese aging population.
The GHABS project also supports and fertilizes new diagnosis methods for early AD diagnosis from academics and pharmaceutical industries.GHABS has been Fig. 5 The general design and goals of the Greater-Bay-Area Healthy Aging Brain Study cohort committed to co-developing early diagnostic tools since its inception, including fluid biomarkers, new antibodies, brain PET instruments, and new PET tracers.In Shenzhen Bay Laboratory, Dr. Qiyu Peng's group are dedicated to developing high-performance and low-cost brain PET/ CT scanner and wearable brain PET/CT scanner [72].The GHABS plans to support the clinical verification of the novel brain PET/CT instruments, as AD is one of the main neurodegenerative diseases that require a braindedicated PET/CT instrument.GHABS is also exploring adjusting the Aβ PET imaging protocols for clinical diagnosis by shortening the scanning time or reducing trace dose using brain PET/CT scanners with high spatial resolution and detection sensitivity.In the future, GHABS will also facilitate AD clinical trials.
In this study, we, for the first time, investigated the abnormal alterations of plasma Aβ 42 , plasma Aβ 42 /Aβ 40 , plasma p-Tau 181 , plasma GFAP, plasma NfL, Aβ PET, tau PET, hippocampal volume, and AD-signature cortical thickness across the different clinical stages of AD based on a Chinese aging cohort in Guangdong-Hong Kong-Macao Greater-Bay-Area.This showed that SCD individuals had significantly higher plasma p-Tau 181 and plasma GFAP than the NC individuals, suggesting that SCD may be related to early increases in plasma p-Tau 181 and plasma GFAP.In addition, CI individuals (MCI or dementia) had abnormal changes in plasma Aβ 42 /Aβ 40 , p-Tau 181 , and NfL, and Aβ PET, tau PET, hippocampal volume, and AD-signature cortical thickness compared to the CU (NC or SCD) individuals.Moreover, the frequencies of plasma Aβ 42 /Aβ 40 positive and plasma p-Tau 181 positive (NC; 5.9%, SCD: 8.2%, MCI: 25.3%, Dementia: 64.9%) and Aβ PET positivity (NC; 25.6%, SCD: 22.5%, MCI: 47.7%, Dementia: 89.3%) were reported for the first time.The overall Aβ-PET positivity rates of NC, MCI, and dementia in C-PAS, an eastern China cohort, were 26.9%, 44.5%, and 85.8%, respectively [73], which was similar to the findings of the GHABS cohort.In general, the frequencies of Aβ PET positivity were in accordance with previous reports [74][75][76].
According to the latest NIA-AA research framework proposed by Jack and colleagues in AAIC 2023, it has been suggested to use a combination of plasma Aβ 42 /Aβ 40 and plasma p-Tau rather than them alone to identify individuals with a high risk of AD.However, our findings indicate that using plasma Aβ 42 /Aβ 40 positive and plasma p-Tau 181 positive (A+/T+) may be only able to capture 23.0%, 36.4%,53.4%, and 72.7% of Aβ PET positivity in NC, SCD, MCI, and dementia individuals respectively.Considering using A-/T + and A+/T-defined by plasma Aβ 42 /Aβ 40 and plasma p-Tau 181 in addition to A+/T + to identify individuals with a high risk of AD, the frequencies of abnormal plasma biomarkers increased by 46.2%, 44.4%, 73.6%, and 93.0% for NC, SCD, MCI, and Dementia respectively.Future investigation is required to determine whether A-/T + or A+/T-individuals defined by plasma Aβ 42 /Aβ 40 and plasma p-Tau 181 have evidence of Aβ plaques, tau tangles, brain atrophy, and cognitive decline.
In summary, we adapt the standard ADNI protocols to collect cognitive assessments, fluid biomarkers, and neuroimaging data to create a community-based observable AD cohort in the Guangdong-Hong Kong-Macao Greater Bay Area of China.The GHABS cohort is expected to identify novel biomarkers and neuroimaging techniques for early detection, determine the appropriate time window for AD intervention, and explore AD's pathological features and progression patterns, especially during the asymptomatic stage of AD in South China's aging population.We reported for the first time the pathophysiology characterization of plasma biomarkers, Aβ PET, tau PET, hippocampal atrophy, and AD-signature cortical thinning, as well as the prevalence of Aβ PET positivity in a Chinese community aging cohort in the Guangdong-Hong Kong-Macao Greater Bay Area of China.These findings provide important guidance towards designing standard AD community cohorts in South China and offer novel insights into understanding the characteristics of abnormal AD pathologies changes in South China's older population.

Fig. 4
Fig. 4 The frequency of plasma A/T profiles and Aβ PET positivity between different clinical groups.Comparisons of (A) A/T profiles defined by plasma Aβ 42 /Aβ 40 and plasma p-Tau 181 , and (B) Aβ PET positivity between NC, SCD, MCI, and Dementia groups

Table 1
Demographics of Greater-Bay-Area Healthy Aging Brain Study (GHABS) participants included in this study